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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Young fibroblast-derived exosomal microRNA-125b transfers beneficial effects on aged cutaneous wound healing

Fig. 7

MiR-125b directly targeted Sirt7. A Predicted binding sites between miR-125b and the Sirt7 3’-UTR. B Dual-luciferase assays were performed in fibroblasts after co-transfection with SIRT7 3’-UTR wild-type (WT) or mutant (MUT) plasmids, and miR-125b mimics or miR-NC mimic. *P < 0.05 versus miR-125b mimic in repeated measures analysis of variance (n = 6) in the WT group. C QRT-PCR for Sirt7 treated with RNA pull down assay. *P < 0.05 versus Control probe in paired t-test (n = 3). D, E Western blot was used to analyze protein level of fibroblasts treated with miR-125b mimic, miR-NC mimic, miR-125b inhibitor, miR-NC inhibitor respectively. *P < 0.05 versus miR-NC mimic; #P < 0.05 versus miR-NC inhibitor in paired t-test (n = 3). F, G Western blot analysis of Sirt7 and β-actin protein levels in fibroblastOld treated with exosomeYoung, exosomeYoung+miR−125b inhibitor or exosomeYoung+miR−NC inhibitor. Untreated fibroblastOld were used as control. *P < 0.05 versus Control; #P < 0.05 versus exosomesYoung+miR−125b inhibitor in repeated measures analysis of variance (n = 6)

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