Fig. 8

Mir-125b/Sirt7 signaling activated migration and FMT of wound-bed fibroblasts ^Old. A–C Fibroblasts^Old were transfected with Ad-Sirt7 or Ad-Ctrl as control. Transfection efficiency was determined using qRT-PCR A and western blot analysis (B, C). ^*P < 0.05 versus Ad-Sirt7 in repeated measures analysis of variance (n = 6). D Images of migrated fibroblasts^Old using Transwell migration assays. (E) Data are presented as the number of migrated cells. F, G Pro-fibrotic genes α-SMA and Col1 mRNA levels were analyzed using qRT-PCR. H Quantification of α-SMA-positive cells. I Representative images of the immunofluorescence staining of α-SMA. J FACS plots detailing the gating strategy to define SMA-positive subpopulations. K Quantification of the relative abundance of SMA-positive cells. ^*P < 0.05 versus Control; ^#P < 0.05 versus exosome^Young + Ad-Sirt7 in repeated measures analysis of variance (n = 6)