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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: GSK-3β suppression upregulates Gli1 to alleviate osteogenesis inhibition in titanium nanoparticle-induced osteolysis

Fig. 3

Blocking GSK-3β activates the Hh-Gli1 signaling pathway and rescues the Ti particle-induced inhibition of osteogenic differentiation. A Protein levels of pSer9-GSK-3β and GSK-3β, compared to 0 μg/cm2. B Protein levels of pSer9-GSK-3β and GSK-3β after TWS119 treatment. C Protein levels of Gli1 in the cytoplasm and nucleus after intervention. D Protein levels of Runx2, Osterix and OCN. EH Gli1, OCN, Osterix and Runx2 mRNA levels. I Representative images of immunofluorescence staining; red (phalloidin), green (Gli1), and blue (nuclei). J Quantitative analysis of the nuclear Gli1 protein ratio. K Quantitative analysis of the average fluorescence intensity of Gli1. In BI, the four groups were cultured in osteogenic medium, and the concentration of Ti particles was 5 µg/cm2. n = 3 per group. *p < 0.05, **p < 0.01, ns: no significance

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