Fig. 6
Hh-Gli1 signaling blockade reversed the upregulation effects of GSK-3β inactivation on Runx2 and OCN expression. A Representative images of immunofluorescence staining; red (phalloidin), green (Runx2 and OCN), and blue (nuclei). B, C Quantitative analysis of the average fluorescence intensity of Runx2 and OCN. The four groups were cultured in osteogenic medium, and the concentration of Ti particles was 5 µg/cm2. n = 3 per group. *p < 0.05, **p < 0.01, ns: no significance