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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Congenital microtia patients: the genetically engineered exosomes released from porous gelatin methacryloyl hydrogel for downstream small RNA profiling, functional modulation of microtia chondrocytes and tissue-engineered ear cartilage regeneration

Fig. 7

3D-Exo activated the PTEN/P13K/AKT/mTOR signaling pathway by delivering exosomal hsa-miR-23a-3p. A The 293 T cells were transfected with luciferase reporter plasmids WT or MUT 3ʹ-UTR of PTEN and hsa-miR-23a-3p mimics/nc, and luciferase activity was detected by Dual-Luciferase Reporter Assay System. B Microtia chondrocytes were treated with 3D-Exo for 48 h, then the expression of hsa-miR-23a-3p in microtia chondrocytes was measured by qPCR. C Western blotting analysis was used for quantifying the protein levels of PTEN, P-PI3K/PI3K, P-AKT/ AKT, and mTOR in microtia chondrocytes. Data were presented as mean ± SD of three number of replicates. ***P < 0.001, ****P < 0.0001, ns: no significance

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Journal of Nanobiotechnology

ISSN: 1477-3155

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