Fig. 3

Characterization and internalization of HUVECs‐Exos. a Morphology of HUVECs-Exos observed by transmission electron microscopy. Scar bar, 100 nm. b Measurement of HUVECs-Exos population by NTA demonstrated a single‐peaked pattern (79.9 ± 0.1 nm in diameter). c Expression of exosome surface markers (CD9, TSG101) was confirmed by western blotting. d Confocal images of human foreskin fibroblasts, HaCAT, HUVECs, and incubated with PBS, 5 ug/ml, 10 ug/ml, 20 μg/ml PKH26-labeled HUVECs-Exos for 24 h. Scar bar, 100 μm