Fig. 3
MiRNA expression profiles in F3- or HFF-derived EVs by small RNA sequencing. A Heatmap showing the differences in the miRNA expression profiles in F3- and HFF-derived EVs (total 92 miRNA). Red signal indicates high expression, and blue signal indicates low expression (normalized log2 values of 8 or more and two-fold change). B Summary diagram of miRNA differentially expressed in F3-derived EVs compared to HFF-derived EVs. C Venn diagram of the number of miRNAs expressed in EVs derived from F3 and HFF cells. The top 10 miRNAs uniquely expressed in F3-derived EVs compared to HFF-derived EVs were listed. D Pie chart of the percentages of involved miRNAs based on significantly changed gene categories. E Bar graph represents the number of miRNAs with significantly increased or decreased expression within the gene categories. F Scatter plot of the comparison of miRNA expression in EVs derived from F3 and HFF cells. Red dots indicate high expression, and green dots indicate low expression. G, H Expression plots of normalized log2 data were displayed in neurogenesis and cell differentiation categories, and in immune and inflammatory response categories. I Expression of selected miRNAs (hsa-mir-20a, hsa-mir-17, hsa-mir183-5p) significantly changed between F3- and HFF-derived EVs was validated using qPCR. (J) GO analysis was conducted for target genes (mRNAs) regulated by top three miRNAs (hsa-mir-20a-5p, hsa-mir-183-5p, hsa-mir-17-5p) that were differentially expressed between F3- and HFF-derived EVs. The GO terms were listed based on p-values (p < 0.05)