Fig. 5
Anti-inflammatory effect of F3-derived EVs in 6-OHDA induced PD mouse model. 6-OHDA (3 mg/mL) was intracerebrally injected with F3- or HFF-derived EVs into the left side of the SN region of the brain. A F3- and HFF-palm-tdTomato-derived EVs (arrows) were observed 24 h after injection of EVs into the SN region of the brain in PD. B, C Reactive astrocytes were identified by GFAP staining in the SN and striatum. The total area of GFAP-positive fluorescence was quantified. D, E Reactive microglia were observed by Iba1 staining in the SN and striatum in a PD mouse model. The total area of the Iba1-positive fluorescence was quantified. F, G RNA expression levels of IL-1α and IL-1β in SN tissues were obtained by qPCR analysis. 6-OHDA-induced reactive glial cells and intracellular inflammatory cytokine levels were reduced by F3-derived EV treatment Scale bar = 50 µm