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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Chimeric virus-like particles presenting tumour-associated MUC1 epitope result in high titers of specific IgG antibodies in the presence of squalene oil-in-water adjuvant: towards safe cancer immunotherapy

Fig. 2

Characterization of chimeric NoV VP1-MUC1 VLPs. A Detection of MUC1 epitope in chimeric NoV VP1-MUC1 produced in L. tarentolae cell lysates; tumour-associated MUC1 peptide served as a positive control, NoV VP1 L. tarentolae cell lysates served as an empty platform control and WT L. tarentolae cell lysates as a background control. The bars represent the mean values obtained from triplicate experiments. B Purification of chimeric NoV VP1-MUC1 VLPs in OptiPrep gradient. Western blotting analysis of the sequential fractions collected from ultracentrifugation in 30–40% gradient. Protein was detected in cell lysates using specific anti-MUC1 antibodies in fractions 6–8. C Electron micrograph of purified L. tarentolae-derived chimeric NoV VP1-MUC1 VLPs (scale bar: 200 nm)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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