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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Chimeric virus-like particles presenting tumour-associated MUC1 epitope result in high titers of specific IgG antibodies in the presence of squalene oil-in-water adjuvant: towards safe cancer immunotherapy

Fig. 4

Analysis of the humoral response induced by NoV VP1-based VLPs in BALB/C mice. Recognition of chimeric NoV VP1-MUC1 particles produced in L. tarentolae by pooled mouse sera collected after vaccination. ELISA plates were coated with serial dilutions of: A recombinant L. tarentolae cell lysates containing chimeric NoV VP1-MUC1 VLPs, B NoV VP1 VLPs (empty platform) or C WT L. tarentolae cell lysates (background threshold). For A-C panels the dilution factor of VLPs or WT lysate is depicted on x-axis. D Comparison of the detection of MUC1 peptide (positive control) and MUC1 epitope in chimeric NoV VP1-MUC1 expressing L. tarentolae cell lysates by MUC1 mouse sera. NoV VP1 L. tarentolae cell lysates served as an empty platform control and WT L. tarentolae cell lysates as a background control. PGM was used as a control of native mucins. For each ELISA assay, the mean from three independent experiments performed is shown. The mean A450 values and standard deviations are shown on the y-axis

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Journal of Nanobiotechnology

ISSN: 1477-3155

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