Fig. 5

Transportation of Fe₃O₄-FAM@M_-BV2 across in vitro BBB. A Schematic diagram of the in vitro BBB. B Transportation ratio of Fe₃O₄-FAM@M_-BV2 across in vitro BBB. C The accumulation of Fe₃O₄-FAM@M_-BV2 in GL261/TR cells after penetrating in vitro BBB detected by LCSM. D The typical flow cytometer diagrams of Fe₃O₄-FAM@M_-BV2 accumulated in GL261/TR cells after Fe₃O₄-FAM@M_-BV2 penetrated in vitro BBB. E Schematic diagram of mechanism study of Fe₃O₄-FAM@M_-BV2 penetrating in vitro BBB. F The effect of CSF-1R, CX3CR1, CSF-1 and CX3CL1 on transportation ratio of Fe₃O₄-FAM@M_-BV2 across the in vitro BBB. (n = 3, mean ± SD, ^*P < 0.05, ^**P < 0.01)