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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: In situ construction of flower-like nanostructured calcium silicate bioceramics for enhancing bone regeneration mediated via FAK/p38 signaling pathway

Fig. 4

Evaluation of the adhesion, spread, and proliferation of BMSCs cultured on CS and nCS bioceramics. a CLSM images of cells cultured for 6 h. Red: actin cytoskeleton (a0, a1), blue: cell nuclei (b0, b1), merge: actin cytoskeleton and cell nuclei (c0, c1). Scale bar = 25 μm. b SEM images of cells cultured for 24 h. (a0, a1) Scale bar = 50 μm; (b0, b1) scale bar = 15 μm; c Immunofluorescence images of BMSCs cultured for 6 h: (a0, a1) cell nuclei were stained blue, (b0, b1) vinculin was stained red, (c0, c1) the actin was stained green, (d0, d1) merged image of the cell nuclei, vinculin, and the cytoskeleton. Scale bar = 50 μm. d MTT quantitative analysis of cells cultured for 1, 3, and 7 days. (*indicates significant differences, p < 0.05)

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