Fig. 1
From: Bacteria-driven hypoxia targeting delivery of chemotherapeutic drug proving outcome of breast cancer
Characterization of DOX-NPs and Bif@DOX-NPs. A Morphological features of BSA-NPs (uncoated albumin nanoparticles) and DOX-NPs (albumin nanoparticles encapsulated with adriamycin) captured by TEM. Scale bar, 100 nm. a: BSA-NPs; b: DOX-NPs. B Average particle size of BSA-NPs and DOX-NPs (n = 3). C Particle size stability of DOX-NPs in DMEM, NS and DW. DMEM Dulbecco's modified eagle medium, NS sanitary saline, DW double-distilled water. D SEM images of naked Bif (a) and Bif@DOX-NPs (b). Scale bar, 1 μm. E Fluorescence spectroscopy analysis of Bif, DOX-NPs and Bif@DOX-NPs. F SDS-PAGE protein analysis of Bif, BSA-NPs and Bif@BSA-NPs, samples were stained with Coomassie Brilliant. G Flow cytometry analysis of Bif and Bif@DOX-NPs. H The relative mean fluorescence intensity (MFI) measured by flow cytometry analysis (n = 3). Asterisks indicate significant differences (****P < 0.0001)