Skip to main content
Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken

Fig. 1

Nbs library construction against S. Enteritidis. a Titer of antibody against S. Enteritidis in the immunized camel serum. b The first round PCR with approximately 700 bp fragment. c The second round PCR with approximately 400 bp fragment. d Nbs library construction counted by tenfold gradient dilution. At the seventh dilution level, 23 single colonies present on the LB plate with 2% final concentration of glucose and 100 µg/mL ampicillin, indicating that 23 phages particles have invaded the TG1 cells, which finally show the phage display library was 2.3 × 109 PFU/mL. e Identification of the correct insert rate of 48 colony by PCR with approximately 700 bp fragment

Back to article page