Skip to main content
Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken

Fig. 6

Development of the double nanobody‑based sandwich ELISA to detect S. Enteriditidis. a The Nb-pair comprising SE-Nb9 and SE-Nb1-vHRP were chosen as capturing and detecting antibodies, respectively. b The concentration of 10 μg/mL SE-Nb1 and SE-Nb1-vHRP (1:50) were optimized for detecting S. Enteriditidis. c The specificity and cross-reactivity analysis with two strains of S. Enteriditidis, three strains of other Salmonella and five other non-Salmonella strains. d The standard curve of the developed immunoassay to detect S. Enteriditidis

Back to article page