Skip to main content

Table 1 Enrichment of phage particles against S. Enteritidis specific nanobodies during four rounds of panning

From: Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken

Round of screening

Input (PFU/well)

P output (PFU/well)

N output (PFU/well)

Recovery(P/Input)

P/N

1st Round

5 × 1011

4.2 × 105

8 × 103

8.4 × 10–7

54

1nd Round

5 × 1011

3.6 × 107

1.2 × 104

7.2 × 10–5

3 × 103

1rd Round

5 × 1011

7.2 × 109

6 × 105

1.44 × 10–3

1.2 × 104

1th Round

5 × 1011

1.2 × 1010

5 × 106

2.4 × 10–2

2.4 × 103