Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken

Table 3 Detection of S. Enteriditidis in skimmed milk after different enrichment period

Sample	Control^a	Enrichment period^b (CFU/mL)
Skimmed milk		6 h	8 h	10 h	12 h	14 h
Skimmed milk	ND^c	ND	1.91 × 10⁵ ± 2.57 × 10⁴	5.07 × 10⁶ ± 4.92 × 10⁵	9.6 × 10⁶ ± 8.73 × 10⁵	2.75 × 10⁷ ± 4.15 × 10⁶

^a‘Control’ is skimmed milk sample without S. Enteriditidis used as blank
^bEach assay was repeated three times, the result shows the average of three replicates
^cND, Not Detectable

ISSN: 1477-3155