Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken

Table 4 Comparisons of the developed sandwich ELISA with real-time PCR and plate culture method by detecting the S. Enteriditidis colonization in challenged chicken

Different assay for detecting S. Enteriditidis	Gastrointestinal tract								Other organs
Different assay for detecting S. Enteriditidis	Stomach	Duodenum	Jejunum	Ileum	Caecum	Colon	Rectum	Pancreas	Heart	Liver	Kidney
Developed sandwich ELISA	−	+	+	+	+	+	+	+	−	+	−
Real-time PCR	+	+	+	+	+	+	+	+	−	+	−
Plate isolation	−	+	+	+	+	+	+	+	−	+	−

Note “−” represents negative for not detectable, “ + ” represents positive for detectable

ISSN: 1477-3155