Fig. 9
The anti-tumor effect of NPs in tumor-bearing mice. A Scheme of the mouse model. K510 cells were subcutaneously injected into nude mice, and tumor engraftment was monitored. When tumor size reached 100 mm3, the different NPs (CEA, CEAM, CEAB, CEAMB) and control PBS were injected into the tail vein on day 1 and day 18. The mice were sacrificed on day 39 for IHC. B The growth curves of tumors. The tumor volume was measured one time every 3 days. (mean ± s.e.m., n = 5). *p < 0.05, (two-tailed t-test). C The tumor volume (mm3) and D the tumor weight (g) was measured at the end of treatment. (mean ± s.e.m., n = 5). *p < 0.05, (two-tailed t-test). NS no significant difference. E The tumors of each group were removed at the endpoint. The dissected slides were used for H&E staining and detecting BCL2, MVP, and CAS3 (Caspase3) protein level by IHC. Scale bar, 100 µm