Fig. 1

GPC3 is specifically expressed on the surface of HCC cells. A, B Immunofluorescence staining of the HCC biomarkers, GPC3 (green) and ARG1 (yellow), in Hep-G2 and THLE-3 cell lines. Scale bars = 100 µm. C, D, E Immunofluorescence staining of the HCC biomarkers, GPC3 (green) and ARG1 (yellow), in fibrotic, cirrhotic, and HCC tissues collected from a CCl₄-treated mouse model of liver cancer. Scale bars = 100 µm. F, G Immunofluorescence staining of the HCC biomarkers, GPC3 (green) and ARG1 (yellow), in human-derived normal liver and HCC tissues. H Quantitative analysis of Immunofluorescence staining of the HCC biomarkers in Hep-G2 and THLE-3 cell lines. I Quantitative analysis of Immunofluorescence staining of the HCC biomarkers in fibrotic, cirrhotic, and HCC tissues collected from a CCl4-treated mouse model of liver cancer. J Quantitative analysis of immunofluorescence staining of the HCC biomarkers in human-derived normal liver and HCC tissues. Scale bars = 100 µm. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001, ns not significant