Fig. 3
From: Nucleophosmin3 carried by small extracellular vesicles contribute to white adipose tissue browning
NPM3 was exclusively localized to sEVs. A Cell immunofluorescence assay confirming the presence of NPM3-EGFP in recipient cells via the transwell assay, GW4869 or Calpeptin was used to block sEVs release, scale bar = 50 μm; B Differential centrifugation procedure for the isolation of sEVs from the supernatants of digested adipose tissue (SN-AT); C The size of different types of EVs (lEVs, mEVs, sEVs) were detected using dynamic light scattering; D NPM3 expressions in different sizes of EVs and different centrifugations supernatants; E Comparison of NPM3 and sEVs marker proteins in 14 fractions (F1–F14) isolated from sucrose density-gradient centrifugation; F Comparison of NPM3, CD63, IGFBP3 in sEVs treated with proteinase K and/or Triton-X-100