Fig. 4

Knocking down NPM3 in BAT impaired sEVs mediated WAT browning. A Relative mRNA expressions of PGC-1α, CIDEA and UCP1 in 3T3-L1 preadipocytes treated with sEVs isolated by TEI or ultracentrifugation method (n = 3), data were represented as mean ± SEM and analyzed by student t test, *P < 0.05,, **P < 0.01; B A flow chart depicting the isolation of sEVs-BAT and sEVs-BAT-siNPM3; C qRT-PCR analysis of thermogenic related mRNA, miRNAs in the sEVs-BAT and sEVs-BAT-siNPM3 (n = 3); D Relative mRNA expressions of PGC-1α, CIDEA and UCP1 in 3T3-L1 preadipocytes treated with sEVs-BAT or sEVs-BAT-siNPM3 (n = 3), data were represented as mean ± SEM and analyzed by student t test, *P < 0.05, **P < 0.01; E Glucose consumption ratio of 3T3-L1 preadipocytes after treated with sEVs-BAT and sEVs-BAT-siNPM3 (n = 3), data were represented as mean ± SEM and analyzed by student t test, *P < 0.05; F Representative UCP1 immunohistochemical staining in iWAT and eWAT in mice treated with sEVs-BAT or sEVs-BAT-siNPM3, Scale bar = 100 μm; G Relative mRNA expressions of PGC-1α, CIDEA and UCP1 in iWAT and eWAT in mice treated with sEVs-BAT or sEVs-BAT-siNPM3 (n = 6), data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, *P < 0.05, **P < 0.01, ***P < 0.001; H Determination of oxygen consumption for mice injected with sEVs-BAT or sEVs-BAT-siNPM3 (n = 3), data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s, *P < 0.05; I Measurement of body temperature for mice injected with sEVs-BAT and sEVs-BAT-siNPM3 under cold treatment over the course of 6 h (n = 4), data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, *P < 0.05, **P < 0.01, ***P < 0.001