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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Nucleophosmin3 carried by small extracellular vesicles contribute to white adipose tissue browning

Fig. 5

Knocking down NPM3 in BAT blunted sEVs mediated insulin sensitivity in HFD-fed mice. A Schematic study plan where 8 weeks old, male C57BL/6 mice were fed with chow or HFD for 15 weeks, HFD-fed mice were injected in vein with sEVs-BAT and sEVs-BAT-siNPM3 during the last 9 weeks; HFD-fed mice treated with PBS were used as controls; B Body weight change during the experiment (n = 6) was evaluated after the intervention, data were represented as meanSEM and analyzed by one-way ANOVA followed by Tukey’s test, **P < 0.01; C Diet intake (Kcal/group); D iWAT, eWAT weight was evaluated after of intervention, data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, **P < 0.01, ***P < 0.001; E H&E staining of iWAT and eWAT were evaluated after of intervention, the size of adipocytes in eWAT of HFD fed mice was evaluated after the intervention (5 images per group), data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, **P < 0.01, ***P < 0.001; F Fasting glucose levels (fasting duration 6 h) were evaluated after the intervention, data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, *P < 0.05, **P < 0.01, ***P < 0.001; G IPGTT and H IPITT were performed in mice (n = 6) after the intervention respectively, data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, P < 0.05, **P < 0.01 versus the HFD group; I Relative mRNA expression of IL-6 and IL-1β in iWAT and eWAT of HFD mice were evaluated after the intervention (n = 6), data were represented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s test, *P < 0.05, **P < 0.01, ***P < 0.001

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