Fig. 3

Cell uptake and intracellular trafficking of liposomal FAM-siRNA. a Flow cytometry measurement of liposomal FAM-siRNA uptake by MCF-7 cells. The FAM-siRNA concentration was 100 nM (n = 3). ***P < 0.001. b Confocal laser scanning microscopy (CLSM) analysis of liposomal FAM-siRNA uptake by MCF-7 cells. The FAM-siRNA concentration was 200 nM. Scale bar, 50 µm. c Intracellular trafficking of FAM-siRNA and the colocalization of FAM-siRNA with early endosomes in MCF-7 cells. The FAM-siRNA concentration was 200 nM. Scale bar, 7.5 µm. d Intracellular trafficking of FAM-siRNA and the colocalization of FAM-siRNA with late endosomes/lysosomes in MCF-7 cells. The FAM-siRNA concentration was 200 nM. Scale bar, 10 µm. Cell nuclei, F-actin, early endosomes and late endosomes/lysosomes were counterstained with Hoechst 33,258 (blue), rhodamine-phalloidin (red), CellLight Early Endosomes-RFP BacMam 2.0 (red) and LysoTracker Red (red), respectively. FAM-siRNA fluorescence (green) was recorded