Fig. 4
In vitro anti-tumor efficacy of MS, ADM-MS and ADM/Fe3O4-MS and the evaluation of ferroptosis. a Live/dead cell staining of MS, ADM-MS and ADM/Fe3O4-MS after co-incubation with LM3 cells. The live cells appeared green, whereas the dead cells appeared red. The scale bar is 100 μm. b Cell viability after treated with Fer-1 (10 μM) and DFO (150 μM) and ADM/Fe3O4-MS with or without microwave irradiation. c Western blot analysis of GPX4 and ACSL4 expression in LM3 cells after the treatment with different formulas. d, e RT-qPCR analysis of GPX4 and ACSL4 gene expression in LM3 cells after the treatment with different formulas