Fig. 2
From: Structure based innovative approach to analyze aptaprobe–GPC3 complexes in hepatocellular carcinoma
Comparative analysis of GPC3 detection with GPC3_3, GPC3_2, and GPC3_9 aptaprobes. A Illustration of aptaprobe-based aptablotting. The 1 μg/mL of aptaprobe was used in aptablotting experiments. B Aptablotting assay of GPC3 protein (lane 1), GPC3 protein spiked in human serum (lane 2), human serum albumin (non-specific negative control, HSA; lane 3), and bovine serum albumin (non-specific negative control, BSA; lane 4). Ponceau S staining was applied to quality control of membrane transfer in aptablotting (C) Aptaprobe-dot blotting assay showing respective signaling of aptaprobes to GPC3, GPC5, H. serum + GPC3 and B. serum + GPC3. GPC3 and GPC5 were serially diluted (100, 50, 10, 1, and 0 pmol) and mixed with complex biological samples (10 μg human/bovine serum). H. serum: human serum; B. serum: bovine serum