Table 1 Sequences and affinity value of GPC3 aptamers
From: Structure based innovative approach to analyze aptaprobe–GPC3 complexes in hepatocellular carcinoma
Clone | Selected sequence | ka (1/Ms) | kd (1/s) | KD (M) |
|---|---|---|---|---|
GPC3_1 | CATGATCAATCCCGTACATATTTTATCCTCAATTTCACA | 2.09 × 104 | 6.45 × 10–5 | 3.09 × 10–9 |
GPC3_2 | ATTTCATAGTGTTCTGTTTTTTTCCAGTTTTCTTATGTCG | 2.28 × 105 | 9.84 × 10–4 | 4.31 × 10–9 |
GPC3_3 | CCTATTCCTTATTATATTTTCTTTTTTTGTAATTTGGTCG | 3.67 × 105 | 4.40 × 10–5 | 1.2 × 10–10 |
GPC3_4 | CCATAATACTGTTTTTTCCGCTGCTATTAGTTACCTCACG | 1.11 × 104 | 7.14 × 10–5 | 6.46 × 10–9 |
GPC3_5 | GCAATCATATGATTTTCCATTACCTCGTTTTTACTTTATT | 2.20 × 105 | 8.53 × 10–4 | 3.88 × 10–9 |
GPC3_6 | TAGTTCTTTTAGGGGTTTTTTGTTTGTCTATGTGTTGTGG | 2.00 × 105 | 1.35 × 10–4 | 6.71 × 10–10 |
GPC3_7 | ATCTTTTAGTTTGTTTGTATATGTTTTTCTCATTTTCTCG | 5.94 × 103 | 6.30 × 10–5 | 1.06 × 10–8 |
GPC3_8 | ATACATTACTATACTGTGCCATTTTTTATATCCGTTCG | 2.75 × 104 | 5.00 × 10–5 | 1.82 × 10–9 |
GPC3_9 | ACTTCCTTTTTTTCGTCTTGTTATCGTTTCCTTATTCTCG | 2.5 × 105 | 5.17 × 10–5 | 2.07 × 10–10 |
GPC3_10 | TTCTTGTCATAAATATTTCCTATTTCCATTCGTACTCCG | 1.72 × 104 | 6.12 × 10–5 | 3.57 × 10–9 |
GPC3_11 | TTTCTTTTGCGTATTATTTACCTTATTTTCTTAGTGTG | 1.80 × 106 | 9.80 × 10–4 | 5.43 × 10–10 |
GPC3_12 | CTTTTTTCGTGTTTTTAGCTCTTTCCGAGTCTTTTCGAAC | 1.09 × 106 | 8.91 × 10–4 | 8.16 × 10–10 |