Fig. 4
a Western blot analysis Cyt c, Bcl-2 and Caspase 3 expression of 4T1 cells. b Quantitative analysis of Cyt c, Bcl-2 and Caspase 3 expression. c Apaf-1 level of cell supernatant after various treatments. P values were calculated by one-way analysis (*p < 0.05, **p < 0.01, ***p < 0.001). Data were represented as mean ± SD (n = 6). d Cytotoxicity profiles of 4T1 cells treated with: (1) PBS, (2) L, (3) FCCF, (4) COF + , (5) FCCF + and (6) OFCCF + . P values were calculated by one-way analysis (*p < 0.05, **p < 0.01, ***p < 0.001). Data were represented as mean ± SD (n = 4). e Schematic diagram of mitochondrial damage induced by bidirectional activation of MPTP opening by Ca2+ and 1O2