Fig. 4

In vivo anti-tumor effects of H-APBC in animal model. a Treatment schedule. b Fluorescence images of mice after intravenous injection of H-APBC and ex vivo imaging of tumor and major organs after 24 h injection. c Photothermal effects of PBS and H-APBC under 808 nm laser irradiation. d Time-dependent manner of temperature change curve in tumor tissues. The representative photographs (e) and weight (f) of tumor tissues in different groups obtained on day 14 (n = 5). g Tumor growth and h body weight of mice in each group. i Fluorescence images of ROS production after different treatments. H&E (j), TUNEL (k), Ki67 (l) staining of tumor tissues in different groups (n = 5). Scale bar: 80 μm. m H&E staining of lung tissues in different groups (n = 5). Scale bar: 100 μm. *p < 0.05, **p < 0.01, and ***p < 0.001