Fig. 3
The growth and differentiation of NE-4C and SH-SY5Y cells after cocultured with PVA, GO/PVA, MoS2/PVA, and MoS2/GO/PVA hydrogels in vitro. Confocal microscopic images of (a) NE-4C cells and b SH-SY5Y cells. Tuj1-positive cells (green) and GFAP-positive cells (red) in different groups (Control, GO/PVA, MoS2/PVA, and MoS2/GO/PVA hydrogels) at 3 and 7 days. All nuclei were stained with DAPI (blue). Scale bar = 50 μm. c Fluorescence intensity values corresponding to confocal photos of each group. d Quantitative of relative gene expression levels of Tuj1, GFAP, ChAT, Islet-1, and HB9 in NE-4C cells in different groups (Control, GO/PVA, MoS2/PVA, and MoS2/GO/PVA hydrogels) at 3 and 7 days. Error bars indicate the SD from four different experiments; *p < 0.05, **p < 0.01, all groups were compared with the control group