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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Engineering defected 2D Pd/H-TiO2 nanosonosensitizers for hypoxia alleviation and enhanced sono-chemodynamic cancer nanotherapy

Fig. 4

SDT and CDT effects of Pd/H-TiO2-PEG at the cellular level. a Bio-TEM images of C6 cells after incubation without and with Pd/H-TiO2-PEG. Scale bars: 1 μm, 0.5 μm and 0.2 μm successively. b Relative viabilities of HUVECs, 4T1 and C6 cells after incubation with elevated concentrations of Pd/H-TiO2-PEG. c Relative viabilities of C6 cells subjected to varied interventions: (1) Control, (2) Pd/H-TiO2-PEG, (3) H2O2, (4) Pd/H-TiO2-PEG + H2O2, (5) US, (6) H2O2 + US, (7) Pd/H-TiO2-PEG + US and (8) Pd/H-TiO2-PEG + H2O2 + US. d Quantitative fluorescent intensity of DCF in different groups. e Fluorescence images of C6 cells stained with DCFH-DA after suffering varied interventions. Scale bars: 25 μm. f Fluorescence images of C6 cells costained with Calcein-AM and PI after suffering varied interventions. Scale bars: 50 μm. g Flow cytometry apoptosis analysis of C6 cells costained with Annexin V and PI after being subjected to varied interventions

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