Fig. 3

Effects of PL, PL-exo and PL-exo-ALN on Dex-induced osteogenesis inhibition in BMSCs. A–B Early osteogenic differentiation was determined by ALP staining and ALP activity assays after 5 days of induction. C–D Late osteogenic differentiation was determined by Alizarin Red staining and the calcium deposition was quantified by measuring the optical density, after 14 days of induction. E–F The expression levels of specific proteins in BMSCs treated as indicated for 3 days. G Immunofluorescence staining of Col-I (green), F-actin(red) and nucleus (blue) after 3 days induction (scale bar: 25 μm). All results are presented as the means ± SDs, ^*P < 0.05, ^**P < 0.01