Fig. 1
Preparation and characterization of the MnCO3-ICG Complexes. A TEM imaging of MnCO3-ICG complexes. B STEM mapping analysis of MnCO3-ICG. C TEM images of MnCO3-ICG after biodegradation in neutral (pH 7.4) and acidic (pH 5.8) PBS for different durations: 0.5, 1, 2, 4 and 8 h. D T1-weighted images of MnCO3-ICG complexes after incubation within different buffer solutions. E Time-dependent T1 intensity change of MnCO3-ICG complexes based on region of interest (ROI) analysis on images from panel D. F Ultrasound images of the generation of CO2 from MnCO3-ICG complexes in acidic buffer solution (pH 5.8 + H2O2) for different time. G Ultrasound signal intensity of the generation of CO2 based on based on ROI analysis on images from panel F. H The decline of absorption peak of MB after MnCO3-ICG incubated at different buffer solutions. I The ESR spectra to detect ·OH produced by MnCO3-ICG, using DMPO as the spin trapper. J Temperature elevation of H2O and different concentrations of MnCO3-ICG ([ICG]: 18, 36 and 72 μg/mL) suspensions under continuous irradiation (808 nm, 0.5 W/cm2, 5 min). K Photothermal heating and natural cooling cycles of ICG, Mn-ICG and MnCO3-ICG ([ICG]: 72 μg/mL, 808 nm, 0.5 W/cm2)