Fig. 2
Ferroptosis mediates IRI in LT with a steatotic donor liver. A Volcano plot and heat map (B) showing the DEGs (Fold change > 1.5; q-value < 0.05;) in the PBS and HM-exo groups (n = 3). C GO analysis of DEGs was carried out, and 10 enriched biological processes are shown. D KEGG analysis of DEGs was carried out, and 10 of the pathways with significant enrichment are shown. E H&E staining images of liver tissue and the liver Suzuki score. F In the Sham group, PBS group, and Fer-1 group (n = 6). G Expression of liver Ptgs2 mRNA in each group, with Actb (β-actin) as the internal reference (n = 6). H Levels of liver MDA and Fe2+ (I) in each group (n = 6). J The level of GPX4 in each group, with GAPDH as the internal reference (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001. LT: Liver transplantation