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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: miR-124-3p delivered by exosomes from heme oxygenase-1 modified bone marrow mesenchymal stem cells inhibits ferroptosis to attenuate ischemia–reperfusion injury in steatotic grafts

Fig. 3

Ferroptosis occurred in H/R treated steatotic IAR20 cells. A Oil red O staining of normal IAR20 cells and steatotic IAR20 cells (S-IAR20). B Steatotic IAR20 cells treated with H/R or Erastin (20 μM), with or without Fer-1 (1 μM) or Emricasan (30 μM), respectively, and CCK-8 assays of cell viability. C The morphology of each group was observed under a phase contrast microscopy, and dead (red) and live (green) cells were identified by PI/FDA staining. D The levels of Lipid-ROS and ROS (E) were detected using C11-BODIPY and DCFH-DA, respectively. F The levels of MDA and Fe2+ (G) in each group of cells (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. ns: not significant

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