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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: miR-124-3p delivered by exosomes from heme oxygenase-1 modified bone marrow mesenchymal stem cells inhibits ferroptosis to attenuate ischemia–reperfusion injury in steatotic grafts

Fig. 4

HM-exos attenuate ferroptosis in steatotic hepatocytes after H/R treatment. A Fluorescence images showing the uptake of CM-DiI-labeled (red) exos by IAR20 and LO2 cells, respectively. B The steatotic IAR20 cells treated with H/R were added to CM or ECM, respectively, and cultured for 6 h. The levels of Lipid-ROS were detected using C11-BODIPY. C The levels of MDA and Fe2+ (D) in cells after CM or ECM intervention. E The steatotic IAR20 cells treated with H/R were added to PBS, exosomes (1.5 × 109 particles), and RNase + Triton-treated exosomes (1.5 × 109 particles), respectively, and cultured for 6 h. The viability of each group of cells was detected using a CCK-8 assay. F PI/FDA staining identifying dead (red) and live (green) cells in each group. G H/R-treated steatotic IAR20 cells were added with PBS, M-exos (1.5 × 109 particles), and HM-exos (1.5 × 109 particles), respectively; exosomes were added during reoxygenation and cultured for 6 h. PI/FDA staining to identify dead (red) and live (green) cells. H The level of Lipid-ROS was detected after intervention with PBS, M-exos, and HM-exos. I The levels of MDA and Fe2+ (J) in cells after intervention with PBS, M-exos, and HM-exos (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. exo: exosomes

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