Fig. 6
HM-exo delivery of miR-124-3p attenuates ferroptosis in H/R-treated cells. A Effect of Erastin on the viability of Steap3-silenced IAR20 cells. B The levels of Lipid-ROS and Fe2+ (C) in Steap3-silenced IAR20 cells after H/R treatment. D Calcein-AM method to determine the LIP levels in each group of cells. E Effect of Erastin on the viability of IAR20 cells overexpressing Steap3. F miR-124-3p mimic and Steap3-OE were transfected into IAR20 cells alone or simultaneously, and the cells’ Lipid-ROS, Fe2+ (G) and LIP (H) levels were measured after H/R treatment. I NC-HM-exos and miR-124-3pin-HM-exos intervention in IAR-20 cells, respectively, followed by treatment with H/R; exosomes were added during reoxygenation and cultured for 6 h, to detect the relative expression of miR-124-3p. J STEAP3 mRNA and protein (K) levels in each group. L Immunofluorescence showing STEAP3 levels in each group. M Lipid-ROS and Fe2+ (N) levels in each group. O PI/FDA staining to identify dead (red) and live (green) cells in each group (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Steap3-OE: Plasmid vector overexpressing Steap3