Fig. 6
Immunomodulation of NETs in vitro. a Fluorescence micrographs of high glucose (50 mM)-treated and LPS-treated neutrophils and stained with SYTOX green on Day 1 and Day 7.The dispersed green area was indicated as NETs (scale bar: 25 μm). b Fluorescence intensity of NETs with high glucose-treated neutrophils. c, d ELISA results for MPO and NE of NETs of high glucose-treated neutrophils. e Representative flow cytometry results after co-culture with LPS, extracts of NETs, extracts of M@M-Ag-Sil-MA7 (the extracts of M@M-Ag-Sil-MA on day 7), or DNase I of RAW264.7.CD 86 and CD 206 represents M1 macrophages surface markers and M2 macrophages surface markers, respectively. f–i ELISA results for TNF-α and IL-10 of high glucose-treated and LPS-treated neutrophils