Fig. 3From: Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca naïve phage display libraryBiophysics of the nanobodies binding to CHIKV E2. a Binding kinetic for nanobodies were obtained by LSPR. b CHIKV E2 protein expression in infected cells was analyzed by immunofluorescence staining using Nb-2E8 and Nb-3C5. c The abilities of Nb-2E8 and Nb-3C5 to block CHIKV E2 interaction with Mxra8 were determined by a competition ELISA. d Cell binding of the nanobodies were quantified by flow cytometry. Left: representative flow cytometry plots; right: bars show mean ± S.E.MBack to article page