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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca naïve phage display library

Fig. 3

Biophysics of the nanobodies binding to CHIKV E2. a Binding kinetic for nanobodies were obtained by LSPR. b CHIKV E2 protein expression in infected cells was analyzed by immunofluorescence staining using Nb-2E8 and Nb-3C5. c The abilities of Nb-2E8 and Nb-3C5 to block CHIKV E2 interaction with Mxra8 were determined by a competition ELISA. d Cell binding of the nanobodies were quantified by flow cytometry. Left: representative flow cytometry plots; right: bars show mean ± S.E.M

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