Fig. 7

A The cell viability of RAW 264.7 cells with different concentrations of AZG and AZGL for 24 h. B The cellular uptake of AZG and AZGL at different points of time under CLSM observation (scale bar = 50 μm). C The generation of intracellular ROS of 4T1 cells with AZL and AZGL according to CLSM images (scale bar = 100 μm). The cell viability of 4T1 cells with different concentrations of D AZL and E AZGL (Data are means ± SD; N = 3). F The cell viability of MDA-MB-231 cells with different concentrations of AZGL (Data means ± SD; N = 3). G Western blot images of HSP90 expression levels with GAPDH as an internal reference for cell lysates of 4T1 cells after different treatments as indicated. H The live/dead staining assay of AZGL with various treatments using Calcein-AM/PI kit (scale bar = 200 μm). I Cell death assay of AZGL with various treatments using Annexin V/PI kit by flow cytometry analysis. Q1 early apoptotic; Q2 secondary necrotic/late apoptotic cells; Q3 necrotic cells; Q4: vital cells