Fig. 6

Evaluation of fibrosis by Masson trichrome staining and Sirius red staining at postoperative Day 28 and quantitative analysis. A Representative images of heart sections stained with Masson’s trichrome and Sirius red (Scale bars of heart cross sections of Masson’s trichrome staining: 1 mm, enlarged images of infarcted area: 100 µm; Sirius red: 100 µm). In Masson staining, collagen was stained blue; in Sirius red staining, the red and yellow tissues observed were considered type I collagen, while the green tissues observed were considered type III collagen. Quantitative analysis of the B ventricular wall thickness, C collagen content, and D collagen I/III ratio in different groups. *P < 0.05 and **P < 0.01, ***P < 0.001