Fig. 6

In vivo immunoregulation ability of Neu-LPs. A Concentration profiles of inflammatory cytokines (TNFα, IL1βand IL6) and chemokine (CXCL2) in ischemic hearts at various timepoints (1, 3, and 7 days) following injection of Neu-LPs (n = 3). B Infiltration of neutrophils in the infarcted area three days after Neu-LP treatment detected by myeloperoxidase (MPO) (n = 3). Scale bars, 100 μm. C Quantitative analysis of MPO-positive cells. D, E Fluorescence images (D) and quantitation (E) of macrophages (F4/80, red) and M2 macrophages (CD206, green) in heart tissue (n = 3) three days after Neu-LP treatment. Scale bars, 100 μm. Statistical analysis was performed using one-way ANOVA. Data are presented as mean ± SD. *P < 0.05 and **P < 0.01 compared with the PBS group