Fig. 3From: Three birds with one stone: co-encapsulation of diclofenac and DL-menthol for realizing enhanced energy deposition, glycolysis inhibition and anti-inflammation in HIFU surgerya Hemolysis test of DC/DLM@PLGA NPs at different concentrations. b MTT and c CCK-8 assays. d Schematic diagram of experimental setup for 4T1 cells exposed to 50% duty cycle HIFU at 25 W. e Cytotoxicity of different DC/DLM@PLGA NPs with gradient concentrations. f Intracellular glucose contents of 4T1 cells and HUVECs after co-incubation with DC/DLM@PLGA NPs for 24 or 48 h. g Live/Dead fluorescent staining of 4T1 cells in PBS, DC/DLM@PLGA NPs, and DC/DLM@PLGA NPs + HIFU groups (60 and 120 s), Scale bar: 100 μm. h Intracellular ATP content measurement and i analysis of HSPs expression after treatment with DC/DLM@PLGA NPs, untreated cell served as a control group. j Schematic representation of the process of DC-induced reduction in protein synthesisBack to article page