Fig. 3
H2 induced apoptosis through mitochondria-dependent pathways. A Calcein AM and PI staining showed confocal fluorescence images of MCF-7 cells with or without xenon lamp irradiation for 10 min. (green: living cells; red: dead cells). Scale bar, 400 μm. B ROS changed in MCF-7 cells after hydrogen treatment. Scale bar, 100 μm. C Fluorescence quantification of ROS. D Detection of MMP changed with JC-1 staining after treatment with H2 for 6 h. Scale bar, 100 μm. E ATP activity and F Caspase-3 released in MCF-7 cells after treatment with H2. Data are presented as the mean ± s.d. (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001