Fig. 5From: Microbial hydrogen “manufactory” for enhanced gas therapy and self-activated immunotherapy via reduced immune escapeHydrogen therapy stimulated immunity in vivo. A The expression of CD86 and MHC II in DC. B Schematic diagram of B16-F10 tumor model. C TNF-α; IFN-γ; IL-1β and IL-6 level in the serum. D Flow cytometry analysis of CD4+ T, CD8+ T cells in mice tumor tissues. E Immunohistochemical and immunofluorescence analysis of CD4+, CD8+, CD161 and Foxp3 in tumor tissues. Scale bar, 50 μm. F Bar graph of CD4+, CD8+, CD161 and Foxp3. Data are presented as the mean ± s.d. (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001Back to article page