Fig. 6From: Microbial hydrogen “manufactory” for enhanced gas therapy and self-activated immunotherapy via reduced immune escapePSB-based H2 therapy avoided immune escape in B16-F10 mice model. A PD-L1 analysis in tumor tissues after different treatments. Scale bar, 50 μm. B Bar graph of quantitation of PD-L1 positive cells. C The expression levels of PD-L1 were determined by tumor tissues with PD-L1 ELISA Kit. D Detection of PD-L1 expression after different treatment with or without TNF-α and PSB. E The expression of PD-L1 was detected by flow cytometry. F Schematic diagram of PD-L1 expression. Data are presented as the mean ± s.d. (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001Back to article page