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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize

Fig. 4

The SPc-delivered ds-MIRNA internalizes into protoplasts and maize through endocytosis. a Differentially expressed genes related to endocytosis in protoplasts. The treated protoplasts were collected after 2 h incubation. The housekeeping gene ZmUBC was used as the reference gene. Triplicate biological replicates were used for each treatment. Different letters on columns indicate significant differences among the treatments of control, ds-MIRNA and ds-MIRNA/SPc complex (Duncan’s multiple range test, P < 0.05). b Differentially expressed genes related to endocytosis in maize. The ds-MIR164b/SPc complex was applied to the root surface of maize every 24 h, and the expression of above endocytosis-related genes was determined on 4 days after the treatment. c Schematic illustration of the ds-MIRNA/SPc complex delivery into cells. The negatively-charged ds-MIRNA can be assembled with positively-charged SPc into ds-MIRNA/SPc complex, which is delivered into cells through endocytosis. The endocytosis-related genes were up-regulated, and the location of encoded proteins is shown

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