Fig. 5

Ds-MIRNA/SPc complex-mediated gene silencing in Arabidopsis. a QRT-PCR assay for MIR166a and miR166a. The ds-MIR166a/SPc complex was applied to the root surface of Arabidopsis every 24 h, and the samples were collected on 6 days after the treatment. The housekeeping gene AtActin-2 was used as the reference gene. Triplicate biological replicates were used for each treatment. Different letters on columns indicate significant differences (Duncan’s multiple range test, P < 0.05). b QRT-PCR assay for target genes. c Photos of Arabidopsis seedlings among various treatments. Scale bar: 500 μm. d Development evaluation of SAM by measuring the leaf # 5 and 6. e The photos of Col (wild type) and transgenic Arabidopsis strain overexpressing Ath-miR166a, which were taken with 10-day-old seedlings. Scale bar: 1 mm