Fig. 3

The protective effects of CsA@PPTK on H/R injured H9c2 cells. A Schematic diagram of the establishment of H/R injured H9c2 cell model. B Representative CLSM image of MitoSOX fluorescence in H/R injury H9c2 cells (red indicates MitoSOX, blue indicates cell nucleus). C Statistical results of MitoSOX fluorescence intensity. D Calcien AM fluorescence intensity measured in H/R injury H9c2 cells by flow cytometer. E Statistical results of Calcien AM fluorescence intensity. F Statistical results of the ratio of red to green fluorescence intensity in H/R injury H9c2 cells after JC-1 staining. n = 3, mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 versus Control group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus H/R group. ^&&&p < 0.001 versus CsA@PTK group