Skip to main content
Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: SHED-derived exosomes promote LPS-induced wound healing with less itching by stimulating macrophage autophagy

Fig. 6

SHED-Exo enhanced autophagy regulates macrophage function. a THP-1 cells treated with 1 μg/mL LPS (LPS), THP-1 treated with LPS and 20 ng/mL exosomes (LPS + Exo), THP-1 treated with LPS, exosomes and 5 mM 3-MA (LPS + Exo + 3MA) and THP-1 treated with LPS and 100 μM rapamycin (LPS + RAPA) for 12 h after PMA stimulation. Representative images of autophagy flux levels in THP-1 cells per treatment group transfected with mRFP‐GFP‐LC3. Scale bars, 10 µm. b Western blot results and relative expression levels of LC3, Beclin1, and P62 proteins per treatment group. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001. c Representative images of mitochondrial membrane potential in THP-1 cells per treatment group as detected by a JC-1 probe. The J-aggregates produced red fluorescence (JC-1 red); the monomer produced green fluorescence (JC-1 green). Scale bars, 20 µm. d Flow cytometry results of ROS levels and mean fluorescent intensity in THP-1 cells per treatment group. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001. e The morphology of mitochondria in THP-1 cells was analyzed by TEM. Scale bars, 1 µm. f Flow cytometry results for surface markers of macrophage polarization

Back to article page